Short-term ovarian histological changes in response to combined Ovaprim™ treatment and intra-ovarian insemination in Barbodes binotatus: Ovaprim-induced ovulation in Barbodes binotatus

Ikmalia Amaliy; Tri Ena  Wulandari; Ragil Angga Prastiya; Darmawan Setia Budi; Nagia Musa Alghoul; Samira Musa Sasi

doi:10.62310/liab.v6i1.387

Authors

Ikmalia Amaliy Master’s Program Student in Reproduction Biology, Faculty of Veterinary Medicine, Universitas Airlangga, Indonesia https://orcid.org/0009-0008-3141-8633
Tri Ena Wulandari Faculty of Health, Medicine and Life Sciences (FIKKIA), Universitas Airlangga, Indonesia
Ragil Angga Prastiya Department of Health and Life Science, Faculty of Health, Medicine and Life Sciences (FIKKIA), Universitas Airlangga, Indonesia https://orcid.org/0000-0003-2658-2099
Darmawan Setia Budi Department of Health and Life Science, Faculty of Health, Medicine and Life Sciences (FIKKIA), Universitas Airlangga, Indonesia https://orcid.org/0000-0001-9508-1437
Nagia Musa Alghoul Libyan Center of Biotechnology Research, Tripoli, Libya
Samira Musa Sasi Department of Zoology, Faculty of science, University of Tripoli, Tripoli, Libya V63C+V67, Tripoli, Libya https://orcid.org/0000-0002-9436-8548

DOI:

https://doi.org/10.62310/liab.v6i1.387

Keywords:

Barbodes binotatus, OvaprimTM, Intra-ovarian insemination, Ovarian histology, Oocyte morphometry, Phase IV oocytes

Abstract

Short-term ovarian responses after a combined hormonal-induction and intra-ovarian insemination procedure have not been characterized in Barbodes binotatus. This exploratory study evaluated ovarian histology, Phase IV oocyte diameter, and Phase IV oocyte-profile counts. Thirty-six gravid females were allocated to a natural-spawning comparator group or a combined Ovaprim™-insemination group (18 per group); three females per group were sampled at 1, 2, 3, 4, 5, and 6 h after insemination or the corresponding comparator time. Individual fish were used as the biological experimental units. Fish-level diameter data were analyzed by two-way ANOVA, whereas Phase IV counts were log(count + 1)-transformed before factorial analysis. No significant differences in oocyte diameters were found among groups (P = 0.427), over time (P = 0.333), or for the group x time interaction (P = 0.824). The combined-procedure group had significantly lower Phase IV profile counts (P = 0.003) while neither time effect (P = 0.197) nor the interaction (P = 0.989)) reached statistical significance. Collapsed follicular remnants were described as post-ovulatory follicle-like structures. Similarly, morphologically basophilic structures compatible with sperm-like material were seen. Ovulation rates, number of released eggs, fertilization, cleavage, hatching, and larval survival were not recorded in the study. Hence the results suggest an association between the combined procedure and certain ovarian-histological patterns but do not confirm treatment-specific causality, reproductive success or an optimal breeding period.

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References

Adolfi MC, Depincé A, Wen M, Pan Q, Herpin A. (2023). Development of ovaries and sex change in fish: Bringing potential into action. Sexual Development 17(2-3): 84-98. https://doi.org/10.1159/000526008

Al Adawiyah L, Sulmartiwi L, Bodur T, Budi DS. (2019). Induction of spermiation using Ovaprim™ with topical gill method in the silver rasbora Rasbora argyrotaenia. Theriogenology 126: 172-176. https://doi.org/10.1016/j.theriogenology.2018.12.014

Ben-Hasan A, Walters C, Hordyk A, Christensen V, Al-Husaini M. (2021). Alleviating growth and recruitment overfishing through simple management changes: Insights from an overexploited long-lived fish. Marine and Coastal Fisheries 13(2): 87-98. https://doi.org/10.1002/mcf2.10140

Bera P, Dhara H, Mandal S, Sau SK, Das S. (2025). Nutritional strategies for improving reproductive performance in fishes. In: Ahmed I, Ahmad I, editors, Aquaculture: Enhancing Food Security and Nutrition. Springer Nature Switzerland, Cham. Pp. 409-430. https://doi.org/10.1007/978-3-031-92858-1_20

Brown-Peterson NJ, Wyanski DM, Saborido-Rey F, Macewicz BJ, Lowerre-Barbieri SK. (2011). A standardized terminology for describing reproductive development in fishes. Marine and Coastal Fisheries 3(1): 52-70. https://doi.org/10.1080/19425120.2011.555724

Chaube R, Singh RK, Joy KP. (2013). Effects of Ovaprim, a commercial spawning inducer, on vasotocin and steroid hormone profiles in the catfish Heteropneustes fossilis: In vivo and in vitro studies. General and Comparative Endocrinology 195: 190-200. https://doi.org/10.1016/j.ygcen.2013.11.009

Corriero A, Zupa R, Mylonas CC, Passantino L. (2021). Atresia of ovarian follicles in fishes, and implications and uses in aquaculture and fisheries. Journal of Fish Diseases 44(9): 1271-1291. https://doi.org/10.1111/jfd.13469

Dobre A, Stroe MD, Lambert P, Dima FM. (2026). Preliminary data regarding the physiological state of Alosa immaculata (Bennett, 1835) during spawning migration. Aquaculture, Fish and Fisheries 6(1): e70188. https://doi.org/10.1002/aff2.70188

Eslamizadeh E, Mabudi H, Roomiani L, Baboli MJ, Dezfulnejad MC. (2024). Effects of thyroxin and luteinizing hormone releasing hormone on reproductive physiology of rohu Labeo rohita: insights into spawning performance, oocyte maturation, steroidogenesis, and follicular development genes. Animal Reproduction Science 267: 107542. https://doi.org/10.2139/ssrn.4761709

Funge-Smith S, Bennett A. (2019). A fresh look at inland fisheries and their role in food security and livelihoods. Fish and Fisheries 20(6): 1176-1195. https://doi.org/10.1111/faf.12403

Gazsi G, Butts IAE, Zadmajid V, Ivánovics B, Ruffilli L, Urbányi B, Csenki Z, Müller T. (2021). Ovarian inseminated sperm impacts spawning success in zebrafish, Danio rerio (Hamilton, 1822) even in the absence of a male stimulus. Theriogenology 172: 315-321. https://doi.org/10.1016/j.theriogenology.2021.07.012

Ittzés I, Kronbauer EC, Szabó T, Horváth L, Urbányi B, Müller T. (2020). Propagation of jundia Rhamdia quelen (Siluriformes: Heptapteridae) by applying the ovarian sperm injection method. Aquaculture Reports 16: 100275. https://doi.org/10.1016/j.aqrep.2020.100275

Mahalingam A, Santhanam P. (2023). Vitellogenesis and reproductive strategies in fishes. In: Baskaralingam V, Vanichviriyakit R, editors, Vitellogenin in Fishes – Diversification, Biological Properties, and Future Perspectives. Springer Nature Singapore, Singapore. Pp. 105-121. https://doi.org/10.1007/978-981-99-5340-0_8

Mujtahidah T, Marsoedi M, Widodo MS. (2019). The reproductive cycle of Puntius binotatus on the middle of the rainy season. Indonesian Journal of Tropical Aquatic 2(1): 9-15. https://doi.org/10.22219/ijota.v2i1.9678

Müller T, Horváth L, Szabó T, Ittzés I, Bognár A, Faidt P, Urbányi B, Kucska B. (2018a). Novel method for induced propagation of fish: Sperm injection in oviducts and ovary/Ovarian lavage with sperm. Aquaculture 482: 124-129. https://doi.org/10.1016/j.aquaculture.2017.09.025

Müller T, Kucska B, Horváth L, Ittzés Á, Urbányi B, Blake C, Guti C, Csorbai B, Kovács B, Szabó T. (2018b). Successful, induced propagation of African catfish (Clarias gariepinus) by ovarian lavage with sperm and hormone mixture. Aquaculture 485: 197-200. https://doi.org/10.1016/j.aquaculture.2017.11.051

Müller T, Ács É, Beliczky G, Makk J, Földi A, Kucska B, Horváth L, Ittzés Á, Hegyi Á, Szabó T, Urbányi B, Quyen NN, Orbán L, Havasi M. (2020). New observations about the fertilisation capacity and latency time of sperm inseminated into the ovary of African catfish (Clarias gariepinus), an oviparous modelfish. Aquaculture 522: 735109. https://doi.org/10.1016/j.aquaculture.2020.735109

Ningrum DRK, Budi DS, Sulmartiwi L. (2019). Induksi ovulasi ikan (Rasbora argyrotaenia) menggunakan Ovaprim™ dengan dosis berbeda. Depik 8(2): 117-124. https://doi.org/10.13170/depik.8.2.14076

Ode I, Rahmadiah T, Abdullah N, Krettiawan H, Alimaturahim F, Rumondang A, Budi DS, Puspaningsih D, Juharni, Nizar M. (2024). Teknologi reproduksi dan pembenihan ikan. Kamiya Jaya Aquatic Press.

Okomoda VT, Amighty RO, Bem TM, Amaantimin J, Nurizzati I, Koh ICC, Abol-Munafi AB, Ikhwanuddin M. (2023). Ovarian lavage method as an alternative route for hormonal administration and short-term sperm storage in Clarias gariepinus. Theriogenology 198: 203-209. https://doi.org/10.1016/j.theriogenology.2022.12.037

Putri NMSA, Pertami ND, Kartika GRA. (2021). Reproduction aspects of spotted barb (Barbodes binotatus Valenciennes, 1842) in Tamblingan lake. Jurnal Sumberdaya Akuatik Indopasifik 5(3): 303-310. https://doi.org/10.46252/jsai-fpik-unipa.2021.vol.5.no.3.159

Reid AJ, Carlson AK, Creed IF, Eliason EJ, Gell PA, Johnson PT, Cooke SJ. (2019). Emerging threats and persistent conservation challenges for freshwater biodiversity. Biological Reviews 94(3): 849-873. https://doi.org/10.1111/brv.12480

Roy A, Barui S, Banerjee R, Das BK. (2025). Hormonal regulation and disorder during fish disease. In: Das BK, Kumar V, editors, Laboratory techniques for fish disease diagnosis. Springer Nature Singapore. Pp. 419-439. https://doi.org/10.1007/978-981-96-4620-3_18

Sahadan FN, Christianus A, Ina-Salwany MY, Ismail FS, Othman R, Zulperi Z. (2022). Gonadotropin-releasing hormone – Its approaches to improve reproduction in fish. Sains Malaysiana 51(11): 3539-3549. https://doi.org/10.17576/jsm-2022-5111-03

Samarin AM, Żarski D, Palińska-Żarska K, Krejszeff S, Blecha M, Kucharczyk D, Policar T. (2017). In vitro storage of unfertilized eggs of Eurasian perch and its effect on egg viability rates and the occurrence of larval malformations. Animal 11(1): 78-83. https://doi.org/10.1017/S1751731116001361

Serrat A, Saborido-Rey F, Garcia-Fernandez C, Muñoz M, Lloret J, Thorsen A, Kjesbu OS. (2019). New insights in oocyte dynamics shed light on the complexities associated with fish reproductive strategies. Scientific Reports 9: 18411. https://doi.org/10.1038/s41598-019-54672-3

Sinjal H. (2014). Efektivitas Ovaprim™ terhadap ovulasi ikan lele. Budidaya Perairan 2(1): 14-21. https://doi.org/10.35800/bdp.2.1.2014.3788

Suryaningsih S, Sagi M, Kamiso HN, Hadisusanto S. (2012). Perubahan kadar hormon testosteron dan progesteron, korelasinya dengan indeks gonado somatik dan tingkat kematangan gonad pada ikan brek (Puntius orphoides Cuvier & Valenciennes, 1842). Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati 17(1). https://doi.org/10.24002/biota.v17i1.129

Tang L, Xiao X, Shi H, Chen J, Han C, Huang H, Li S. (2020). Induction of oocyte maturation and changes in the biochemical composition, physiology and molecular biology of oocytes during maturation and hydration in the orange-spotted grouper (Epinephelus coioides). Aquaculture 522: 735115. https://doi.org/10.1016/j.aquaculture.2020.735115

Yaron Z, Gur G, Melamed P, Rosenfeld H, Elizur A, Levavi-Sivan B. (2003). Regulation of fish gonadotropins. International Review of Cytology 225: 131-185. https://doi.org/10.1016/S0074-7696(05)25004-0

Zohar Y, Muñoz-Cueto JA, Elizur A, Kah O. (2010). Neuroendocrinology of reproduction in teleost fish. General and Comparative Endocrinology 165: 438-455. https://doi.org/10.1016/j.ygcen.2009.04.017