Comparison of Fluorescence Polarization Assay with Rose Bengal Plate Agglutination test and Competitive Enzyme-Linked Immunosorbent assay for bovine brucellosis in Tanzania
Comparative diagnosis of Brucellosis in Tanzania
Keywords:Brucellosis , Rose Bengal Plate Agglutination test, c-Enzyme-Linked Immunosorbent Assay, Fluorescence Polarisation Assay
Brucella are Gram-negative, facultative, intracellular bacterial species with B. abortus, B. melitensis, and B. suis carrying the smooth-lipopolysaccharide antigen. Accurate diagnostic results of brucellosis are needed for its control and eradication, however, they are primarily based on the serological testing of brucellosis in animals. The Rose Bengal Plate Agglutination Test (RBPT) and competitive Enzyme-Linked Immunosorbent Assay (c-ELISA) are the most commonly used tests for making such diagnosis. The use of Fluorescence Polarization Assay (FPA) in Tanzania is still in nascent stage. The purpose of this study was to compare RBPT, c- ELISA, and FPA in the diagnosis of bovine brucellosis. A total of 75 serum samples from cattle that were infected with Brucella in Kagera region were obtained. The FPA showed 90% (68 samples) prevalence, RBPT revealed 93% (70 samples) prevalence, and c-ELISA revealed 81% (61 samples) prevalence of brucellosis in the farm. The RBPT test has shown an inability to distinguish antibodies from cross-reacting organisms compared to the FPA test, while the c- ELISA was unable to pick a positive sample compared to the FPA test. FPA is very quick (5 min per sample), does not require specialized staff, and may be performed under field conditions. Therefore, FPA has a potential to overcome limitations in the detection of bovine brucellosis and can be used as a confirmatory test.
Cardoso PG, Macedo CG, Azevedo V, Oliveira CS. (2006). Review, Brucella spp non canonical LPS: structure, biosynthesis and interaction with host immune system; Microbial Cell Factories 5: 13. https://doi.org/10.1186/1475-2859-5-13
Corbel MJ, Gill KPW, Thomas EL. (1983). Methods for the identification of Brucella. Central Veterinary Laboratory, New Haw, Wey bridge, UK. Pp. 1–63.
Foster G, MacMillan AP, Godfroid J, Howie F, Ross HM, Cloeckaert A, Reid RJ, Brew S, Patterson IAP. (2002). A review of Brucella sp. infection of sea mammals with particular emphasis on isolates from Scotland. Veterinary Microbiology 36: 563-580.
Gall D, Nielsen K, Forbes L, Davis D, Elzer P, Olsen S, Balsevicius S, Kelly L, Smith P, Tan S, Joly D. (2000). Validation of the fluorescence polarization assay and comparison to other serological assays for the detection of serum antibodies to Brucella abortus in bison. Journal of Wildlife Diseases 36: 469-476.
Gall D, Nielsen K. (2004). Serological diagnosis of bovine brucellosis: a review of test performance and cost comparison. Revue Scientifique et Technique – OIE 23(3): 989-1002.
Godfroid J, Saegerman C, Wellemansa V. (2002). How to substantiate eradication of Bovine brucellosis when a specific serological reactions occur in the course of brucellosis testing. Veterinary Microbiology 90: 461-477.
Gwida MM, El-Gohary AH, Melzer F, Tomaso H, Rosler U, Wernery U, Wernery R, Elschner CM, Khan I, Eickhoff M, Schoner D, Neubauer H. (2011). Comparison of diagnostic tests for the detection of Brucella spp. in camel sera. BMC Research Notes 4: 525. https://doi.org/10.1186/1756-0500-4-525
Lucero NE, Escobar GI, Ayala SM, Silva Paulo P, Nielsen K. (2003). Fluorescence polarization assay for diagnosis of Human brucellosis. Journal of Medical Microbiology 52: 883-887.
Mc Given JA, Stack JA, Perrett LL, Tucker JD, Brew SD, Stubberfield E, MacMillan AP. (2006). Harmonisation of European tests for serological diagnosis of infection in bovines. Revue Scientifique et Technique – OIE 25(3): 1039-1053.
Mc Given JA, Tucker JD, Perrett LL, Stack JA, Brew SD, MacMillan AP. (2003). Validation of FPA and c-ELISA for the detection of antibodies to Brucella abortus in cattle sera and comparison to SAT, CFT, and iELISA. Journal of Immunological Methods 278: 171-178.
Minas A, Stournara A, Minas M, Stack J, Petridou E, Christodoulopoulos G, Krikelis V. (2007). Validation of a fluorescence polarization assay (FPA) performed in microplates and comparison with other tests used for diagnosing B. melitensis infection in sheep and goats. Journal of Immunological Methods 320, 94-103.
Muma JB, Lund A, Nielsen K, Matope G, Munyeme M, Mwacalimba K, Skjerve E. (2009). Effectiveness of Rose Bengal test andfluorescence polarization assay in the diagnosis of Brucella spp. Infections in free range cattle reared in endemic areas in Zambia. Tropical Animal Health and Production 41: 723-729.
Munoz P, Marin CM, Monreal D. (2004). Efficacy of several serological tests and antigens for diagnosis of Cattle brucellosis in the presence of false positive serological results due to Yersinia enterocolitica O:9. Clinical and Diagnostic Laboratory Immunology 12: 141-151.
Nielsen K, Gall D, Bermudek R, Renteria T, Moreno F, Corral A, Monroy O, Monge F, Smith P, Widdison J, Mardrueno M, Calderon N, Guerrero N, Tinoco R, Osuna J, Kelly W. (2002). Field trial of the brucellosis fluorescence polarization assay. Journal of Immunoassay and Immunochemistry 23: 307-316.
Nielsen K, Gall D, Smith P, Kelly W, Yeo J, Kenny K, Heneghan T, McNamara S, Maher P, O’Connor J, Walsh B, Carroll J, Rojas X, Rojas F, Perez B, Wolff O, Bufoni L, Salustio E, Gregoret R, Samartino L, Dajer A, Luna-Martinez E. (2001). Fluorescence polarization assay for the diagnosis of Bovine brucellosis: adaptation to field use. Veterinary Microbiology 80: 163-170.
Nielsen K, Smith P, Widdison J, Gall D, Kelly L, Kelly W, Nicoletti P. (2004). Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O:157:H7. Veterinary Microbiology 100: 25-30.
Nielsen K, Smith P, Yu W, Nicoletti P, Elzer P, Robles C, Bermudez R, Renteria T, Moreno FS, Ruiz A, Massengill C, Muenks Q, Jurgersen G, Tollersrud T, Samartino L, Conde S, Forbes L, Gall D, Perez B, Rojas X, Minas A. (2005). Towards single screening tests for brucellosis. Revue Scientifique et Technique - OIE 24(3): 1027-1037.
OIE. (2008). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, OIE, Paris, France.
OIE. (2011). Bovine brucellosis. In: Manual of diagnostic tests and vaccines for terrestrial animals. World Organization for Animal Health, Paris, France. Pp. 1-35.
Weiner M, Iwaniak W, Zlotnicka J, Szulowski K. (2010). Diagnosis of Bovine brucellosis using traditional serological techniques and Fluoresence polarization assay. Bulletin of the Veterinary Institute in Pulawy 54: 485-488.
How to Cite
Copyright (c) 2023 All the papers accepted for publication in LIAB will be published as open access.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
All the papers accepted for publication in LIAB will be published as open access.