Assessing probiotic attributes and molecular characterization of Weissella confusa from barbari goat faeces using 16S rRNA: Probiotic attributes and molecular characterization of Weissella confusa

Prashant  Pandey; Ajit  Kumar; Harshit Verma; Debashis  Roy; Mrinal Srivastava; Malyaj R.  Prajapati; Shriya  Rawat; Atul  Gupta; Nishant  Sharma

doi:10.62310/liab.v5i2.242

Authors

Prashant Pandey College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250110
Ajit Kumar College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250110
Harshit Verma College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250110 https://orcid.org/0000-0002-0104-7754
Debashis Roy College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250110
Mrinal Srivastava College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250110
Malyaj R. Prajapati College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250110 https://orcid.org/0000-0002-8542-9732
Shriya Rawat College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250110 https://orcid.org/0009-0001-1546-9405
Atul Gupta College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250110 https://orcid.org/0009-0006-7471-2301
Nishant Sharma College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250110

DOI:

https://doi.org/10.62310/liab.v5i2.242

Keywords:

Weissella confusa, Probiotics, 16S rRNA, Goat faeces, Lactic acid bacteria, Molecular identification

Abstract

The present study examined the probiotic potential and molecular characterization of Weissella confusa isolated from goat faeces using 16S rRNA gene sequencing. A total of 15 lactic acid bacterial isolates were obtained from 42 fresh faecal samples collected from healthy barbari goats. Based on morphological, biochemical, and molecular analyses, two isolates goat faecal-7 (GF-7) and goat faecal-8 (GF-8) (Genbank accession numbers: MZ314055.1 Weissella confusa India and MZ314055.2 Weissella confusa India) were identified as W. confusa with 99.2% similarity to reference sequences in GenBank. The isolates demonstrated significant probiotic attributes including acid tolerance (survival at pH 2.5), bile salt resistance (0.3% oxgall), adhesion to intestinal epithelial cells (34.2%), and antimicrobial activity against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. Antibiotic susceptibility testing revealed sensitivity to ampicillin, chloramphenicol, and tetracycline, indicating their safety for potential probiotic applications. The strains produced lactic acid at a concentration of 8.4 g/L and exhibited β-galactosidase activity. Phylogenetic analysis confirmed that the isolates belong to the Weissella genus. The results indicate that W. confusa MZ314055.1 and MZ314055.2 possess probiotic characteristics suitable for functional food development and animal health enhancement.

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