Freezing ability of two slow permeating cryoprotectants on rooster semen diluted with tris egg-yolk juice extender: Cryopreservation of rooster semen

Adedeji Suleimon Balogun; Raman Narang; Ranjna S Cheema; Prem Prakash Dubey; Shakti kant  Dash; Neeraj  Kashyap; Saroj Kumar Sahoo

doi:10.62310/liab.v1i2.61

Authors

Adedeji Suleimon Balogun Department of Animal Production Technology, Oyo State College of Agriculture and Technology, PMB10, Igboora, Oyo State, Nigeria https://orcid.org/0000-0002-6116-7459
Raman Narang Department of Animal genetics and Breeding, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Science University, Punjab, India
Ranjna S Cheema Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Science University, Punjab, India https://orcid.org/0000-0003-4509-7339
Prem Prakash Dubey Directorate of livestock farms, Guru Angad Dev Veterinary and Animal Science University, Punjab, India
Shakti kant Dash Directorate of livestock farms, Guru Angad Dev Veterinary and Animal Science University, Punjab, India https://orcid.org/0000-0001-9658-3017
Neeraj Kashyap Department of Animal genetics and Breeding, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Science University, Punjab, India
Saroj Kumar Sahoo Directorate of livestock farms, Guru Angad Dev Veterinary and Animal Science University, Punjab, India https://orcid.org/0000-0002-3966-2496

DOI:

https://doi.org/10.62310/liab.v1i2.61

Keywords:

Quil egg-yolk, Cryopreservation, Cryoprotectants, Antioxidants, Fertility, Chicken

Abstract

Cryo-damage reduction with slow permeating cryoprotectants has been identified as a promising approach for poultry semen preservation. This study was conducted to evaluate the efficacy of dimethylformamide (DMF), methylacetamide (MA), and their mixtures (DMF+MA) in the cryopreservation of rooster semen extended with tris quail egg-yolk (TEY) extender supplemented with orange or tomato juice. The extender was prepared by adding 25% of egg-yolk in tris buffer. 18% & 13% (v/v) DMF, MA, and (DMF+MA) were added to the extender constituting six treatments. Semen was collected from fifteen roosters of 35weeks of age by backstroke massage of mid abdomen followed by semen pooling. Pooled semen was divided into six fractions and two steps dilution was done. Extended semen samples were subjected to cryopreservation procedures and finally stored at -196 oC. After 48h, semen samples were thawed at 5oC and evaluated for motility, viability, membrane integrity, and acrosome integrity. Effects of juices on TEY extended semen were further evaluated with 13% and 18% (DMF+MA). 15% of orange (O) and tomato (T) juices were supplemented to TEY extender. Same dilution, freezing and evaluation procedures were observed. Finally, fertility trials were conducted on the best treatments. The results revealed that 13% and 18% (DMF+MA) had significantly (P<0.05) higher motility, viability, and membrane integrity. TEYO-18% (DMF+MA), TEYO 13%(DMF+MA+O), and TEYT 13% (DMF+MA) had significantly (P<0.05) higher motility and membrane integrity compared to others. 100% fertility rate was also recorded for 13%(DMF+MA). It was concluded that 13% (DMF+MA) with or without juice supplementation is beneficial

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