Freezing ability of two slow permeating cryoprotectants on rooster semen diluted with tris egg-yolk juice extender

Cryopreservation of rooster semen

Authors

  • Adedeji Suleimon Balogun Department of Animal Production Technology, Oyo State College of Agriculture and Technology, PMB10, Igboora, Oyo State, Nigeria https://orcid.org/0000-0002-6116-7459
  • Raman Narang Department of Animal genetics and Breeding, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Science University, Punjab, India
  • Ranjna S Cheema Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Science University, Punjab, India https://orcid.org/0000-0003-4509-7339
  • Prem Prakash Dubey Directorate of livestock farms, Guru Angad Dev Veterinary and Animal Science University, Punjab, India
  • Shakti kant Dash Directorate of livestock farms, Guru Angad Dev Veterinary and Animal Science University, Punjab, India https://orcid.org/0000-0001-9658-3017
  • Neeraj Kashyap Department of Animal genetics and Breeding, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Science University, Punjab, India
  • Saroj Kumar Sahoo Directorate of livestock farms, Guru Angad Dev Veterinary and Animal Science University, Punjab, India https://orcid.org/0000-0002-3966-2496

DOI:

https://doi.org/10.62310/liab.v1i2.61

Keywords:

Quil egg-yolk, Cryopreservation, Cryoprotectants, Antioxidants, Fertility, Chicken

Abstract

Cryo-damage reduction with slow permeating cryoprotectants has been identified as a promising approach for poultry semen preservation. This study was conducted to evaluate the efficacy of dimethylformamide (DMF), methylacetamide (MA), and their mixtures (DMF+MA) in the cryopreservation of rooster semen extended with tris quail egg-yolk (TEY) extender supplemented with orange or tomato juice. The extender was prepared by adding 25% of egg-yolk in tris buffer. 18% & 13% (v/v) DMF, MA, and (DMF+MA) were added to the extender constituting six treatments. Semen was collected from fifteen roosters of 35weeks of age by backstroke massage of mid abdomen followed by semen pooling. Pooled semen was divided into six fractions and two steps dilution was done. Extended semen samples were subjected to cryopreservation procedures and finally stored at -196 oC. After 48h, semen samples were thawed at 5oC and evaluated for motility, viability, membrane integrity, and acrosome integrity. Effects of juices on TEY extended semen were further evaluated with 13% and 18% (DMF+MA). 15% of orange (O) and tomato (T) juices were supplemented to TEY extender. Same dilution, freezing and evaluation procedures were observed. Finally, fertility trials were conducted on the best treatments. The results revealed that 13% and 18% (DMF+MA) had significantly (P<0.05) higher motility, viability, and membrane integrity. TEYO-18% (DMF+MA), TEYO 13%(DMF+MA+O), and TEYT 13% (DMF+MA) had significantly (P<0.05) higher motility and membrane integrity compared to others. 100% fertility rate was also recorded for 13%(DMF+MA). It was concluded that 13% (DMF+MA) with or without juice supplementation is beneficial 

Metrics

Metrics Loading ...

References

Balogun AS, Jimoh OA, Ojo JA, Oke TD, Akinosun AA (2015). Assessment of selected fresh garlic extracts antioxidant potentials for mitigating reactive oxygen species (ROS) in diluted rooster semen used for artificial insemination. Book of abstract of Humboldt Conference, Alexander Von Humboldt Foundation 2-7 August, Osun State University, Osogbo, Osun State, Nigeria, Pp. 28.

Beirao J, Robles V, Herraez MP, Sarasquete C, Dinis MT, Cabrita E (2006). Cryoprotectant microinjection toxicity and chilling sensitivity in gilthead seabream (Sparus aurata) embryos. Aquaculture 261: 897-903

Blanco JM, Long JA, Gee G, Wildt DE, Donoghue AM (2012). Comparative cryopreservation of avian spermatozoa: effects of freezing and thawing rates on turkey and sand hill crane sperm cryo-survival. Animal Reproduction Science 131: 1-8.

Blesbois E, Grasseau I, Seigneurin F, Mignon-Grasteau S, Saint Jalme M, Mialon-Richard MM (2008). Predictors of semen cryopreservation in chickens. Theriogenology 69: 252-261.

Blesbois E, Seigneurin F, Grasseau I, limouzin C, Bernard J, Gourichon D, Coquerelle G, Rault P, Tixier-boichard M (2007). Semen cryopreservation for ex situ management of genetic diversity in chicken: creation of the French avian cryobank. Poultry Science 86: 555-564.

Cmhughes R, Lewis SE, McKelvey VJ, Thompson W (1998). The effects of antioxidant supplementation during percoll preparation on human sperm DNA integrity. Human Reproduction 13: 1240-1247.

Ehling C, Taylor U, Baulain U, Weigend S, Henning M, Rath D (2012). Cryopreservation of semen from genetic resource chicken lines. Agriculture and Forestry Research 3(62): 151-158.

Eilish TD, Neil M, Sheena EML (1999). The effect of ascorbate and α- tocopherol supplementation in vitro on DNA integrity and hydrogen peroxide induced DNA damage inhuman spermatozoa. Mutagenesis 14: 505-512.

Elansary E, Elsharawy G, Asar M, Waleed F (1999). Effect of ascorbic acid on semen characteristics of Alexandria cockerels under hot ambient temperatures. Poultry Science 78: 35, Abstract.

Fahy GM, Lilley TH, Linsdell H, Douglas MJ, Meryman HT (1990). Cryoprotectant toxicity and cryoprotectant toxicity reduction in search of molecular mechanisms. Cryobiology 27: 247-268.

Fairchild BD, Christensen VL, Grimes JL, Wineland MJ, Bagley LG (2002). Hen age relationship with embryonic mortality and fertility in commercial turkeys. Journal of Applied Poultry Research 11: 260-265.

Hanzawa S, Ninomi T, Takahashi R, Yamaguchi K, Miyata T, Tajima A (2006). New method of freezing chicken semen using N- methyl-acetamide as cryo-protecting agent. In: Romboli I, Flock DK, Franchini A, editors, 12th European Poultry Conference, 10-14 September, Verona, Italy.

Hiemstra SJ, Van Der Lende T, Woelders H (2005). The potential of cryopreservation and reproductive technologies for animal genetic resources conservation strategies. In: Ruane J, Sonnino A, editors, The role of biotechnology in exploring and protecting agricultural genetic resources, FAO, Rome, Italy.

Khan RU, Rahman ZU, Javed I, Muhammad F (2012). Effect of vitamins, probiotics and protein on semen traits in post-molt male broiler breeders. Animal Reproduction Science 135: 85-90.

Niemann H (1991). Cryopreservation of ova and embryos from livestock: current status and research needs. Theriogenology 35: 109-124.

Ortega-Ferrulosa C, Sotillio-Galan Y, Varela-Fernandez E, Gallardo- Bolanos JM, Muriel A, Gonzalez-Fer-nandez L, Tapia JA, Pena FJ (2008). Detection of “apoptosis-like” changes during the cryopreservation process in equine sperm. Journal of Andrology 29: 213-221.

Parks EJ, Graham JK (1992). Effects of cryopreservation procedures on sperm membranes. Theriogenology 38: 209-222.

Said TM, Gaglani A, Agarwal A (2010). Implication of apoptosis in sperm cryoinjury. Reproductive BioMedicine Online 21: 456-462.

Sasaki K, Tatsumi T, Tsutsui M, Niinomi T, Imai T, Naito M, Nishi Y (2010). A method for cryopreserving semen from Yakido roosters uning N-methylacetamide as a cryoprotective agent. The Journal of Poultry Science 47: 297-301.

Shanmugam M, Mahapatra RK (2017). Pellet cryopreservation of chicken semen: Effect of dimethyl acetamide and sucrose on post- thaw sperm quality and fertility. In: National symposium on “biodynamic animal farming for the management of livestock diversity under changing global scenario”. XIV Annual convention of society for Conservation of Domestic Animal Biodiversity, 8-10 February, Thrissur, Kerala, India Pp. 158.

Surai PF, Bortolotti GR, Fidgett A, Blount J, Speake BK (2001). Effects of piscivory on the fatty acid profiles and antioxidants of avian yolk: studies on eggs of the gannet, skua, pelican. and cormorant. Journal of Zoology London 255: 305-312.

Swain JE, Smith GD (2010). Cryoprotectants. In: Chian RC, Quinn P, editors, Fertility cryopreservation. Cambridge University Press, New York, USA, Pp. 24-38.

Tselutin K, Seigneurin F, Blesbois E (1999). Comparison of cryoprotectants and methods of cryopreservation of fowl spermatozoa. Poultry Science 78: 586-590.

Downloads

Published

02-12-2021

How to Cite

Balogun, A. S., Narang, R., Cheema, R. S., Dubey, P. P., Dash, S. kant ., Kashyap , . N. ., & Sahoo, S. K. (2021). Freezing ability of two slow permeating cryoprotectants on rooster semen diluted with tris egg-yolk juice extender: Cryopreservation of rooster semen. Letters In Animal Biology, 1(2), 07–13. https://doi.org/10.62310/liab.v1i2.61

Issue

Section

Research Articles
Recieved 2021-10-21
Accepted 2021-11-30
Published 2021-12-02

Most read articles by the same author(s)