Optimizing liquid storage duration of two poultry species semen with plant based extender: Semen preservation in poultry

Adedeji Suleimon Balogun

doi:10.62310/liab.v1i2.62

Authors

Adedeji Suleimon Balogun Department of Animal Production Technology, Oyo State College of Agriculture and Technology, PMB10, Igboora, Oyo State, Nigeria https://orcid.org/0000-0002-6116-7459

DOI:

https://doi.org/10.62310/liab.v1i2.62

Keywords:

Semen preservation, Plant-based extender, Semen quality, Turkey, Chicken

Abstract

Liquid storage of semen preservation for poultry species is deemed to be the only reliable and cost effective method compare to cryopreservation. This experiment was conducted to examine the preservation capacity of Tris coconut-water orange juice extender (TCWOE) on tom and cock semen for artificial insemination. TCWOE was prepared. Semen ejaculates were collected and pooled from five toms and cocks separately. The pooled semen from each species was divided into two portions and in one portion of each species the extender was added in the ratio of 1:3 (semen: extender), making a total of four treatments. Experimental design used was a complete randomized design consisting of three trials. Semen microscopic parameters like motility, viability, membrane integrity, and acrosome integrity were examined and recorded for freshly extended semen and preserved semen at 4-8oC every 12 h interval till 72 h. The motility and membrane integrity of freshly extended cock semen was significantly higher (P<0.05) compared to motility of extended tom semen and membrane integrity of un-extended tom semen. During storage up to 24 h, extended tom and cock semen showed significantly higher (P<0.05) motility and membrane integrity compare to un-extended semen. Extended cock semen had significant (P<0.05) higher percentage motility, livability and membrane integrity compared to un-extended and extended tom semen from 36 h to 72 h of storage. However, no significant difference (P>0.05) was observed for acrosome integrity. Conclusively, cock semen survived longer and had better results considerable for artificial insemination than tom semen up to 72 h storage. However, the extender was beneficial for storage of both species semen.

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References

Balogun AS (2019). Evaluation of antioxidant potentials of tomato & orange juices on extended poultry semen preserved with egg-yolk & coconut water diluents to improve selection intensity. Ph.D Dissertation. Guru Angad Dev Veterinary and Animal Science University, Ludhiana, Punjab, India.

Balogun AS, Jimoh OA, Mustapha A, Mohammed A, Akinosun AA, Ojo JA (2018). Assessment of different garlic extracts on fertilizing potentials of extended rooster semen used for artificial insemination. Nigeria Poultry Science Journal 12: 84-91.

Balogun AS, Jimoh OA, Akinosun AA, Oluwemimo BA. (2016). Efficacy of egg-yolk citrate extender fortified with aqueous garlic extract on rooster semen. Proceeding of the 41st Annual Conference, Nigerian Society for Animal Production (NSAP) 20-24 March, Federal University of Agriculture Abeokuta, Ogun State, Pp. 155-157.

Balogun AS, Shivkumar, Mustapha A, Fatola OSG, Imtiyaz N (2017a). Spermogram and fertility assessment of cocks’ semen, extended with coconut-water extender supplemented with garlic extracts. Role of livestock in sustainable agriculture. in: Proceedings of IAHA pre- conference on organic animal husbandry linked to the 19th Organic World Congress, New Delhi, India, November 9-11, pp. 190-195.

Balogun AS, Shivkumar, Akinosun AA, Fatola OSG, Oluwemimo BA (2017b). The cold storage capability of coconut- water supplemented with garlic extracts on roosters’ semen. Role of livestock in sustainable agriculture. in: Proceedings of IAHA pre-conference on organic animal husbandry linked to the 19th Organic World Congress, New Delhi, India, November 9-11, Pp. 170-174.

Balogun AS, Jimoh O A, Ojo JA, Oke TD, Akinosun AA (2015). Assessment of selected of selected fresh garlic extracts antioxidant potentials for mitigating reactive oxygen species (ROS) in diluted rooster semen used for artificial insemination. Book of abstract of Humboldt Conference, Alexander Von Humboldt Foundation 2 – 7 August, Osun State University, Osogbo, Osun State, Nigeria, Pp. 28.

Blanco JM, Gee G, Wildt DE, Donoghue L (2000). Species variation in osmotic cryoprotectant, and cooling rate tolerance in poultry, eagle and peregrine falcon spermatozoa. Biology of Reproduction 63: 1164−1171.

Blanco JM, Long JA, Gee G, Donoghue AM, Wildt DE (2008). Osmotic tolerance of avian spermatozoa: influence of time, temperature, cryoprotectant and membrane ion pump function on sperm viability. Cryobiology 56, 8–14.

Blesbois E, Grasseau I, Seigneurin F (2005). Membrane fluidity and the ability to survive cryopreservation in domestic bird spermatozoa. Reproduction 129: 371–378.

Chaveiro A, Liu J, Engel B, Critser JK, Woelders H (2006). Significant variability among bulls in the sperm membrane permeability for water and glycerol: possible implications for semen freezing protocols for individual males. Cryobiology 53, 349–359.

Donoghue AM, Wishart GJ (2000). Storage of poultry semen. Animal Reproduction Science 62: 213–232

Diaz JD, Corrada Y, PG Blanco, Gobello C (2013). In vitro and in vivo assessment of skim milk with and without egg yolk on canine spermatozoa incubated at 4 0C. Animal Reproduction 10(4): 670-676.

Hafez B and Hafez ESE (2000). Reproduction in Farm Animals. 7th ed. New York. Lippincott Williams and Wilkins, USA.

Iaffaldano N, Dilorio M, Cerolini S, Manchisi M (2016). Overview of turkey semen storage: focus on cryopreservation – a review. Annals of Animal Science 16(4): 961–974.

Iaffaldano N, Manchisi A, Rosato MP (2008). The preservability of turkey semen quality during liquid storage in relation to strain and age of males. Animal Reproduction Science 109: 266–273.

Kasai k, Izumo A, Inaba T, Sawada T (2000). Assessment of fresh and stored duck spermatozoa quality via in vitro sperm-egg interaction assay. Theriogenology 54: 283–290.

King LM, Holsberger DR, Donoghue AM (2000). Correlation of CASA velocity and linearity parameters with sperm mobility phenotype in turkeys. Journal of Andrology 21(1): 65-71.

Lemoine M, Grasseau I, Magistrini M, Blesbois E (2011). Ability of chicken spermatozoa to undergo acrosome reaction after liquid storage or cryopreservation. Theriogenology 75: 122–130.

Leahy T, Gadella BM (2011). Sperm surface changes and physiological consequences induced by sperm handling and storage. Reproduction 142, 759-778.

Linde-Forsberg C (1995). Artificial insemination with fresh, chilled, extended and frozen thawed semen in the dog. Seminars in Veterinary Medicine and Surgery (Small Animal), 10(1): 48-58.

Penfold L, Harna lV, Lynch W, Bird D, Derricson S, Wildt E (2001). Characterization of northern pintail (Anas acuta) ejaculate and the effect of sperm preservation on fertility. Reproduction 121: 267–275.

Roca J, Hernandez M, Carvajal G, Vazquez JM, Martinez EA (2006). Factors influencing boar sperm cryosurvival. Journal of Animal Science 84, 2692–2699.

Sexton TJ (1974). Oxidative and glycolytic activity of chicken and turkey spermatozoa. Comparative Biochemistry and Physiology B 48: 39–65.

Tabatabaci S, Batavani RA, Talcbi AR (2009). Comparison of semen quality in indigenous and Ross broiler breeder roosters. Journal of Animal and Veterinary Advances 8(1), 90-93.

Wishart GJ (2009). Semen quality and semen storage. In: Biology of breeding poultry. Poultry Science Symposium Series, Hocking P. (ed.). London, UK, pp. 151–178

Yu I, Songsasen N, Godke RA, Leibo SP (2002). Differences among dogs in response of their spermatozoa to cryopreservation using various cooling and warming rates. Cryobiology 44, 62–78.