Inflection in nutrient transporter genes leads to potential changes in small intestine histomorphology with improved nutrient retention in chicken under dietary synbiotic supplementation: Synbiotic supplementation and nutrient transporter expression

Kapil Dev; Jitendra  Singh; Nasir Akbar Mir; Avishek Biswas; Joyshikh  Sonowal; Rajiv  Kant; Neeraj  Ahlawat; Ashok Kumar Tiwari

doi:10.62310/liab.v2i1.75

Authors

Kapil Dev ICAR-Central Avian Research Institute, Izatnagar, Bareilly-243122, India
Jitendra Singh CSIR-Central Drug Research Institute, Lucknow-226031, India
Nasir Akbar Mir ICAR-Central Avian Research Institute, Izatnagar, Bareilly-243122, India
Avishek Biswas ICAR-Central Avian Research Institute, Izatnagar, Bareilly-243122, India
Joyshikh Sonowal ICAR-Indian Veterinary Research Institute, Izatnagar Bareilly-243122, India
Rajiv Kant Sam Higginbottom University of Agriculture, Technology And Sciences, Prayagraj (Allahabad)-211007, India
Neeraj Ahlawat Sam Higginbottom University of Agriculture, Technology And Sciences, Prayagraj (Allahabad)-211007, India
Ashok Kumar Tiwari ICAR-Central Avian Research Institute, Izatnagar, Bareilly-243122, India

DOI:

https://doi.org/10.62310/liab.v2i1.75

Keywords:

Synbiotics, Nutrient transporters, Gut histology, Nutrient digestibility, Broiler chicken

Abstract

This study investigated the role of synbiotic supplementation on gut histomorphology, expression of nutrient transporters, and nutrient utilization in broiler chicken. Bacitracin methylene disalicylate (BMD), Probiotic Lactobacillus acidophilus (LBA), and prebiotic Mannan-oligosaccharides (MOS) were used to formulate total 6 dietary treatments viz. T1 (control; basal diet), T2 (BMD @ 20 mg/kg diet), T3/T4 (0.1% MOS with 106 and 107 cfu LBA/g diet), and T5/T6 (0.2% MOS with 106 and 107 cfu LBA/g diet). A total of 288 day old chicken were allocated at random among six treatments, each with six replicates of eight chicken (48 birds/treatment). Results revealed higher villus height, crypt depth, villus height: crypt depth ratio, and histological surface magnification ratio values in chicken fed a combination of MOS (0.2%) and LBA (106 or 107 cfu/g diet). BMD supplemented birds had higher values of these parameters compared to control birds. The villus width, villus bottom area, and mucosal unit bottom area were all increased in birds supplemented with BMD, but were similar to birds fed a combination of MOS (0.1 percent) and LBA (107 cfu/g diet). The villus width, villus bottom area, and mucosal unit bottom area were higher in BMD supplemented birds which were similar to the birds fed a combination of MOS (0.1%) and LBA (107 cfu/g diet). The birds fed a combination of MOS (0.2%) and LBA (106 or 107 cfu/g diet) revealed upregulation of SGLT1 and GLUT5 expression in jejunum but no significant effect was observed on the expression of PePT1 and EAAT3 gene. The AME Diet, nitrogen, phosphorus, and calcium retentions were higher in birds fed a combination of MOS (0.2%) and LBA (106 or 107 cfu/g diet). However, the organic matter digestibility was higher in birds fed a combination of MOS (0.2%) and LBA (106 cfu/g diet) and BMD supplementation also improved the organic matter utilization compared to control. In conclusion, the supplementation of a combination of MOS (0.2%) and LBA (106 or 107 cfu/g diet) improves the intestinal architecture along with upregulation of SGLT1 and GLUT5 nutrient transporters and increase in nutrient digestibility in broiler chicken.

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References

Agboola AF, Aroniyo I, Suberu SA and Adeyemi WT. (2014). Dietary supplementation of probiotics and synbiotics on intestinal microbial populations and gut morphology of turkey poults. African Journal of Livestock Extension 14: 13-20.

Alloui MN, Szczurek W. and Swiatkiewicz S. (2013). The Usefulness of Prebiotics and Probiotics in Modern Poultry Nutrition: a Review/Przydatnosc prebiotykow i probiotykow w nowoczesnym zywieniu drobiu-przeglad. Annals of Animal Science 13(1): 17.

Amat C, Planas JM and Moreto M. (1996). Kinetics of hexose uptake by the small and large intestine of the chicken. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 271(4): R1085-R1089.

AOAC (1990). Official Methods of Analysis. 15th Edn. Association of official analytical chemists. Washington D.C. 20044.

Awad WA, Ghareeb K, Abdel-Raheem S, Böhm J. (2009). Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poultry Science 88(1), 49-56.

Begum J, Buyamayum B, Lingaraju MC, Dev K. (2021). Probiotics: Role in immunomodulation and consequent effects. Letters in Animal Biology 01(01): 01-06.

BIS (2007). Nutrient requirements for poultry 13: 9863, Bureau of Indian Standards, New Delhi.

Chen H, Pan Y, Wong EA, Bloomquist JR and Webb Jr KE. (2002). Molecular cloning and functional expression of a chicken intestinal peptide transporter (cPepT1) in Xenopus oocytes and Chinese hamster ovary cells. The Journal of Nutrition 132(3):387- 393.

Daniel H. (2004). Molecular and integrative physiology of intestinal peptide transport. Annual Review of Physiology 66:361-384.

Douard V and Ferraris RP. (2008). Regulation of the fructose transporter GLUT5 in health and disease. American Journal of Physiology-Endocrinology and Metabolism 295(2): E227-E237.

Fan YK, Croom J, Christensen VL, Black BL, Bird AR, Daniel LR and Eisen EJ. (1997). Jejunal glucose uptake and oxygen consumption in turkey poults selected for rapid growth. Poultry Science 76(12):1738-1745.

Gaggìa F, Mattarelli P. and Biavati B. (2010). Probiotics and prebiotics in animal feeding for safe food production. International Journal of Food Microbiology 141: S15-S28.

Ghahri H, Toloei T. and Soleimani B. (2013). Efficacy of antibiotic, probiotic, prebiotic and synbiotic on growth performance, organ weights, intestinal histomorphology and immune response in broiler chickens. Global Journal of Animal Scientific Research 1(1):25-41.

Gilbert ER, Li H, Emmerson DA, Webb Jr KE and Wong EA. (2007). Developmental regulation of nutrient transporter and enzyme mRNA abundance in the small intestine of broilers. Poultry Science 86(8):1739-1753.

Gourbeyre P, Denery S and Bodinier M. (2011). Probiotics, prebiotics, and synbiotics: impact on the gut immune system and allergic reactions. Journal of Leukocyte Biology 89(5):685-695.

Iwanaga T, Goto M and Watanabe M. (2005). Cellular distribution of glutamate transporters in the gastrointestinal tract of mice. An immunohistochemical and in situ hybridization approach. Biomedical Research 26(6):271-278.

Kanai Y and Hediger MA. (2004). The glutamate/neutral amino acid transporter family SLC1: molecular, physiological and pharmacological aspects. Pflügers Archiv - European Journal of Physiology 447(5):469-479.

Kim JS, Ingale SL, Kim YW, Kim KH, Sen S, Ryu MH and Chae BJ. (2012). Effect of supplementation of multi‐microbe probiotic product on growth performance, apparent digestibility, cecal microbiota and small intestinal morphology of broilers. Journal of Animal Physiology and Animal Nutrition 96(4):618-626.

Kisielinski K, Willis S, Prescher A, Klosterhalfen B and Schumpelick V. (2002). A simple new method to calculate small intestine absorptive surface in the rat. Clinical and Experimental Medicine 2(3):131-135.

Kocher AN. and Tucker L.U. (2005). The ‘gut health’response to dietary Bio-Mos®: effects on gut microbiology, intestinal morphology and immune response. In Nutritional Biotechnology in the Feed and Food Industries. Proceedings of Alltech’s 21st Annual Symposium (Eds TP Lyons and KA Jacques). Nottingham University Press, UK (pp. 383- 388).

Miles RD, Butcher GD, Henry PR and Littell RC. (2006). Effect of antibiotic growth promoters on broiler performance, intestinal growth parameters, and quantitative morphology. Poultry Science 85(3):476-485.

Mohan B, Kadirvel R, Natarajan A and Bhaskaran M. (1996). Effect of probiotic supplementation on growth, nitrogen utilisation and serum cholesterol in broilers. British poultry science 37(2):395-401.

Mountzouris KC, Tsirtsikos P, Kalamara E, Nitsch S, Schatzmayr G and Fegeros K. (2007). Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poultry Science 86(2):309-317.

Mountzouris KC, Tsitrsikos P, Palamidi I, Arvaniti A, Mohnl M, Schatzmayr G, Fegeros K. (2010) Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition. Poultry science 89:58-67.

Nisar H, Sharif M, Rahman MA, Rehman S, Kamboh AA, Saeed M. (2021). Effects of Dietary Supplementations of Synbiotics on Growth Performance, Carcass Characteristics and Nutrient Digestibility of Broiler Chicken. Brazilian Journal of Poultry Science 23(2): 01-10.

Pelicano ERL, Souza PA, Souza HBA, Figueiredo DF, Boiago MM, Carvalho SR and Bordon VF (2005). Intestinal mucosa development in broiler chickens fed natural growth promoters. Brazilian Journal of Poultry Science 7:221-229.

Pfaffl MW, Horgan GW and Dempfle L. (2002). Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research 30: e36. https ://doi.org/10.1093/nar/30.9.e36

Salim HM, Kang HK, Akter N, Kim DW, Kim JH, Kim MJ. and Kim WK. (2013). Supplementation of direct-fed microbials as an alternative to antibiotic on growth 207 performance, immune response, cecal microbial population, and ileal morphology of broiler chickens. Poultry Science 92(8):2084-2090.

Sharifi SD, Dibamehr A., Lotfollahian H. and Baurhoo B. (2012). Effects of flavomycin and probiotic supplementation to diets containing different sources of fat on growth 208 performance, intestinal morphology, apparent metabolizable energy, and fat digestibility in broiler chickens. Poultry Science 91(4):918-927.

Shim YH, Shinde PL, Choi JY, Kim JS, Seo DK, Pak JI and Kwon IK. (2010). Evaluation of multi-microbial probiotics produced by submerged liquid and solid substrate fermentation methods in broilers. Asian-Australasian Journal of Animal Sciences 23(4): 521-529.

Steel A, Nussberger S, Romero MF, Boron WF, Boyd CA, Hediger MA. (1997). Stoichiometry and pH dependence of the rabbit proton‐dependent oligopeptide transporter PepT1. The Journal of Physiology 498(3):563-569.

Talapatra SK, Ray SC and Sen KC. (1940). The analysis of mineral constituents in biological materials. 1. Estimation of phosphorus, chlorine, calcium, magnesium, sodium and potassium in food-stuffs. Indian Journal of Veterinary Science 10:243-258.

Teshfam M, Rahimi S and Karimi K. (2005). Effect of various levels of probiotic on morphology of intestinal mucosa in broiler chicks. 205-211.

Tuohy KM, Probert HM, Smejkal CW, Gibson GR (2003). Using probiotics and prebiotics to improve gut health. Drug discovery today 8:692- 700.

Vineetha PG, Tomar S, Saxena VK, Kapgate M, Suvarna A and Adil K. (2017). Effect of laboratory‐isolated Lactobacillus plantarum LGFCP 4 from gastrointestinal tract of guinea fowl on growth performance, carcass traits, intestinal histomorphometry and gastrointestinal microflora population in broiler chicken. Journal of Animal Physiology and Animal Nutrition 101(5):e362-e370

Wang X, Farnell YZ, Peebles ED, Kiess AS, Wamsley KGS and Zhai W. (2016). Effects of prebiotics, probiotics, and their combination on growth performance, small intestine morphology, and resident Lactobacillus of male broilers. Poultry Science 95(6): 1332-1340.

Wright EM and Turk E. (2004). The sodium/glucose cotransport family SLC5. Pflügers Archiv 447(5):510-518.

Yang Y, Iji PA, Kocher A, Mikkelsen LL, Choct M. (2008). Effects of dietary mannanoligosaccharide on growth performance, nutrient digestibility and gut development of broilers given different cereal‐based diets. Journal of animal physiology and animal nutrition 92:650-659.

Yang YING, Iji PA, Kocher A, Thomson E, Mikkelsen LL and Choct M. (2008). Effects of mannan-oligosaccharide in broiler chicken diets on growth performance, energy utilisation, nutrient digestibility and intestinal microflora. British Poultry Science 49(2): 186-194.

Yun JS, Seo DS, Kim WK and Ko Y. (2005). Expression and relationship of the insulin-like growth factor system with posthatch growth in the Korean Native Ogol chicken. Poultry Science 84(1): 83-90.

Yun W, Lee DH, Choi YI, Kim IH, Cho JH. (2017). Effects of supplementation of probiotics and prebiotics on growth performance, nutrient digestibility, organ weight, fecal microbiota, blood profile, and excreta noxious gas emissions in broilers. Journal of Applied Poultry Research 26:584-592.